Vimentin anticorps

N° du produit ABIN955516

Cet anticorps Poulet Polyclonal détecte spécifiquement Vimentin dans WB, IF et IHC (p). Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour Vimentin anticorps (ABIN955516)

Antigène: Vimentin (VIM)

Reactivité: Humain, Souris

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp Vimentin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Vimentin anticorps

Specificité: Recognizes Vimentin.

Purification: Affinity Chromatography using a peptide column.

Immunogène: Chickens were immunized with three synthetic peptide KLH conjugated corresponding to different regions of the Vimentin gene product, but are shared between the Human (NP_003371) and Mouse (NP_035831) sequences. Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Isotype: Ig Fraction

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.3 mg/mL

Buffer: 10 mM PBS, pH 7.2 containing 0.02 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted in the dark at 2-8 °C.

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: Vimentin

Sujet: Human vimentin is a 53,521 dalton protein (466 amino acids) expressed in many cell types of mesodermal origin, and is one of the intermediate filament types that form the cytoskeleton. In the embryonic nervous system, however, vimentin is expressed by migrating neural crest cells forming the PNS, and by neural stem cells of the CNS. In some forms of glioblastoma, vimenin is reexpressed by the tumor cells and serves as a general indicator of their undifferentiated state.Synonyms: VIM

ID gène: 7431

NCBI Accession: NP_003371

Pathways: Caspase Cascade in Apoptosis